NettetHoechst 33342 Staining Dye Solution (ab228551) is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be used in fluorescence microscopy, microplate, cuvette, and flow cytometry applications. It can also be used to detect the contents of a sample DNA by plotting a standard emission-tocontent curve. NettetChemical Analysis, Life Sciences, and Diagnostics Agilent

Do I need to permeabilize cells for Hoechst staining?

NettetSaccharomyces cerevisiae stained with the FUN 1 cell stain, which generates red-fluorescent intravacuolar structures, and with Calcofluor White M2R, a blue-fluorescent cell wall stain, both components of the LIVE/DEAD Yeast Viability Kit. LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry evaluates membrane integrity Nettet23. okt. 2024 · Staining your floors is a major decision for three reasons: 1) Your choice will have a substantial impact on the overall look of your home, 2) You will be living with … dick powell movies youtube

Labeling Nuclear DNA with Hoechst 33342 - CSH Protocols

NettetMechanism of cell death was evaluated by chromatin condensation using Hoest staining and morphological changes with the use of a contrast microscope. Plant extracts of G. asiatica were observed to induce apoptosis of MCF-7 cells as evidenced by MTT-cell proliferation assay, cell-morphological changes and chromatin condensation. Nettetendobj xref 683 44 0000000016 00000 n 0000001859 00000 n 0000002024 00000 n 0000004021 00000 n 0000004048 00000 n 0000004185 00000 n 0000004789 00000 n … NettetOne advantage of Hoechst 33342 is that it is membrane permeant and, thus, can stain live cells. Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. citroen hy van fish and chips