WebApr 12, 2024 · Mice were orally administered with B-mEVs starting at 4 weeks feeding on MCD diet 1.2 mg/kg every other day for 4 weeks. Saline or EV-depleted supernatant (1.2 mg/kg every other day) were used as negative control. Mice were kept on a 12-hour light and dark cycle. All animal experiments were approved by the Institutional Animal Care … WebMale B6C3F1 mice were administered styrene monomer by oral gavage for 29 consecutive days at dose levels of 0, 75, 150, or 300 mg/kg/day. The highest dose level represented the maximum tolerated dose based on findings in a 28-day dose range-finding study, in which the bioavailability of orally administered styrene was also confirmed.
Alteration of microbiota and immune response of mice …
WebJan 27, 2024 · Introduction, this video protocol will detail the setup and procedure for gavaging probiotics to a neonatal mouse, on it's day of life two. The sterile collection of intestines, for intestinal microbiome analysis, is also detailed in this protocol. Some representative results, are also included in this protocol, to introduce some of the types ... WebKong. Conventional (male C57BL/6) mice were given azoxy-methane to induce colon neoplasia after receiving a course of antibiotics in drinking water. Mice were gavaged twice weekly with stool from 5 patients with CRC or 5 healthy individuals (controls) for 5 weeks. Germ-free C57BL/6 mice were gavaged once with stool from 5 patients with CRC or 5 ... hennepin healthcare pediatric gender clinic
Stable engraftment of human microbiota into mice with a single …
WebIn a study approved by The Telethon Kids Institute Animal Ethics Committee (approval #232), we examined the effects of repeated gavage on respiratory outcomes. Female … Web2. The position of the mouse is very important. Align the head and body vertically with the esophagus. 3. Never force the feeding needle down, allow the mouse to swallow and … WebMalassezia restricta exacerbates acute ethanol-induced liver injury in mice. Wild type mice were gavaged once with M. restricta or PBS followed by a dose of ethanol (300 µL, 30% vol/vol) or an isocaloric amount of dextrose (300 μL, 0.42 g/mL) 1 hour later, and harvested after 9 hours. A, Serum levels of ALT. B, Hepatic triglyceride content. C and D, Hepatic … larry smith contractors cleves oh