WebMar 19, 2024 · For such a bioenergetics assay, use injection port A for the drug of interest and move the injections corresponding to the mitochondrial or glycolysis stress test to port B, C and D respectively (Figure 3 D). An example of such an application in a mitochondrial stress test, can be found in our recent study (Ludikhuize et al., 2024). Note that ... WebAug 28, 2024 · formed using XFp Cell Mito Stress Test Kit (Cat. No. 103010-100; Agilent Technologies), XFp Cell Energy Phenotype Test Kit (Cat. No. 103275-100; Agilent Technologies), XFp Mito Fuel Flex Test Kit (Cat. No. 103270-100; Agilent Techno-logies), and Substrate Oxidation Stress Tests (Cat. No. 103672-100 for Seahorse XF Long …
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WebSep 1, 2024 · I've been following the MitoStress Test protocol for T cells provided by Agilent. Does anyone have tips and tricks to rein-in the errors bars. Mine are outrageously large. Things that I have tried ... WebMar 3, 2024 · Start Mito Stress assay with Seahorse XF Cell Mito Stress Test Kit (#103015-100, Agilent Technologies). Strictly follow the manufacturers’ manual (Mito_Stress_Test_Kit_Guide). With this system, oxygen consumption rate (OCR) is quantified real-time after sequential addition of 2 mM Oligomycin, 1 mM FCCP and 5 mM … co z remote programming
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WebJan 7, 2024 · The Cell Mito Stress Test is a common method to measure the key parameters of mitochondrial respiration. Here, we use the human cell line HK-2 as an example to present the procedures to quantify the oxygen consumption rate using a Seahorse XFe96 extracellular flux analyzer. WebNov 2, 2024 · Fatty acid oxidation: Fatty acid oxidation was measured by the XF Fatty Acid Oxidation assay combined with the Cell Mito Stress Test (Agilent Technologies, Santa Clara, CA, USA) to determine whether fatty acid oxidation contributed to the measured OCR. This assay was performed according to the manufacturer’s protocol, with some … WebJan 7, 2024 · The Cell Mito Stress Test is a common method to measure the key … co zrobila fagata